Figure 8.

SOX4 was detected to express more in tumor tissues than the normal

(A) The enrichment of SOX4, DAB2IP, DGKH, RASA1, MSN, and CBL using RT-PCR in the sample pulled down by biotinylated miR-338-3p. ∗∗p < 0.01, compared with biotinylated NC (Bio-NC). Data indicate the mean ± SD. n = 3, repetition = 3. (B) The relative expression of SOX4 mRNA was detected by RT-PCR in normal (n = 51) and tumor tissues (n = 51). GAPDH was used as the reference. (C) Relative expression of SOX4 mRNA in cell lines (Nthy-ori3-1, Htori-3, K1, TPC-1, CAL-62, IHH-4) was analyzed by RT-PCR. GAPDH was used as a standard. (D) Relative expression of SOX4 protein in cell lines (Nthy-ori3-1, Htori-3, K1, TPC-1, CAL-62, IHH-4) was analyzed by western blot. GAPDH was used as a standard. (E) The correlation between miR-338-3p and SOX4 mRNA expression in PTC tissues was determined by Spearman’s correlation analysis. ∗p < 0.05, ∗∗p < 0.01, compared with Nthy-ori 3-1. Data indicate the mean ± SD. n = 3, repetition = 3.