Figure 5.

A functional J-domain in Ydj1 and Sis1 is required for enhancing the Ssa1-to-Sgt2 TA transfer.A and C, representative Western blot images of Bos1^Bpa transfer from Ssa1 to Sgt2 in the presence of the indicated Ydj1 (A) or Sis1 (C) variants. Reactions were carried out as described in Figure 3A. B and D, quantification of the efficiency of the Bos1-Sgt2 crosslink from replicates of the data in (A) and (C), respectively-. E and G, representative Western blot image of the time courses of Bos1^Bpa transfer from Ssa1(T201A) to Sgt2 in the absence and presence of Ydj1 (E) or Sis1 (G). F and H, quantification of the efficiency of the Bos1-Sgt2 crosslink from replicates of the data in (E) and (G), respectively. All values in (D), (F), and (H) are reported as mean ± SD, with n ≥ 3. Error bars are shown but may not be visible in some cases. The lines in (B) represent the mean from two independent measurements. TA, tail-anchored protein.