KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Polyclonal anti-HspQ	This study	N/A
Polyclonal anti-DnaA	(Sutton and Kaguni, 1995)	PMID: 7592447
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa 680	Invitrogen	A-21076
Bacterial Strains
Y. pestis KIM5–3001 (parent)	(Jackson et al., 2004)	PMID: 15554975
Y. pestis KIM5–3001 Δlon	(Jackson et al., 2004)	PMID: 15554975
dnaA508 tnaA::Tn10	(Hinds and Sandler, 2004)	PMID: 15588282
dnaA508 tnaA::Tn10 lon::kan	This study	N/A
BL21(DE3) Star™	Invitrogen	C601003
Chemicals, Peptides, and Recombinant Proteins
NADH, disodium salt	Roche	10107735001
Pyruvate Kinase from rabbit muscle	Sigma	P9136
Lactate Dehygrogenase, from rabbit muscle	Calbiochem	427217
Creatine Kinase from rabbit muscle	Roche	10736988001
Adenosine-5′triphosphate (ATP)	Roche	10127531001
Creatine Phosphate	Roche	10621722001
Phosphoenolpyruvate potassium salt	Bachem	4014027.0005
Oligonucleotides
hspQYP105 (Forward): 5′-gagcatatgatcgccagcaaattcggtatag-3′ hspQYP105 (Reverse): 5′-caggagctcttagttacgtaggtgcggagcc-3′	This study	N/A
ymoA (Forward): 5′-tgtacatatgacaaaaactgactacc-3′ ymoA (Reverse): 5′-agaggatccttatttcacatgttgccatac-3′	This study	N/A
y2853 (Forward): 5′-tacttccaatccaatgcaatggattcactcatcgtccctg-3′ y2853 (Reverse): 5′ttatccacttccaatgttattaatgaatgatggggtaagaggtgg-3′	This study	N/A
hspQEC (Forward): 5′-gtgcatatgattgccagcaaattcggtatc-3′ hspQEC (Reverse): 5′-gttgagctcttagttacgcagacgcgggg-3′	This study	N/A
hspQ95 (Top): 5′-gatgaactggcggcctcgattcgctaacagttacaggctccgcacctacg-3′ hspQ95 (Bottom): 5′-cgtaggtgcggagcctgtaactgttagcgaatcgaggccgccagttcatc-3’	This study	N/A
hspQDD (Top): 5′-ccagttacaggctccgcacctagatgactaagagctccgtcgacaagc-3′ hspQDD (Bottom): 5′-gcttgtcgacggagctcttagtcatctaggtgcggagcctgtaactgg-3	This study	N/A
hspQK5D (Top): 5′-ggcagccatatgatcgccagcgatttcggtataggccagcagg-3′ hspQK5D (Bottom): 5′-cctgctggcctataccgaaatcgctggcgatcatatggctgcc-3′	This study	N/A
hspQK5E (Top): 5′-ggcagccatatgatcgccagcgaattcggtataggccagcagg-3′ hspQK5E (Bottom): 5′-cctgctggcctataccgaattcgctggcgatcatatggctgcc-3′	This study	N/A
hspQK5R (Top): 5′-ggcagccatatgatcgccagccgtttcggtataggccagcagg-3′ hspQK5R (Bottom): 5′-cctgctggcctataccgaaacggctggcgatcatatggctgcc-3′	This study	N/A
hspQD25N (Top): 5′-cgggtatttgggagtggtgattaatatcgatcctgaatattctcttgc-3′ hspQD25N (Bottom): 5′-gcaagagaatattcaggatcgatattaatcaccactcccaaatacccg-3′	This study	N/A
hspQD27N (Top): 5′-gggagtggtgattgatatcaatcctgaatattctcttgcacc-3′ hspQD27N (Bottom): 5′-ggtgcaagagaatattcaggattgatatcaatcaccactccc-3′	This study	N/A
hspQS93A (Top): 5′-ccttcattagatgaactggcggccgcgattcgccaccagttacaggctcc-3′ hspQS93A(Bottom): 5′-ggagcctgtaactggtggcgaatcgcggccgccagttcatctaatgaagg-3′	This study	N/A
hspQRHQ/AAA (Top): 5′-gatgaactggcggcctcgattgccgccgcgttac-3′ hspQRHQ/AAA (Bottom): 3′-gttacgtaggtgcggagcctgtaacgcggcggcaatc-5′	This study	N/A
hspQext (Top): 5′-caaccttcattagatgaactggcggcctcgattcgcggcagcaccggcagcaccggcagc-3′ hspQext (Bottom): 3′-ctcttagttacgtaggtgcggagcctgtaactggtggctgccggtgctgccggtgctgcc-5′	This study	N/A
Recombinant DNA
pET28b-hspQEC	This study	N/A
pET28b-hspQYP	This study	N/A
pET28b-hspQ95	This study	N/A
pET28b-hspQDD	This study	N/A
pET28b- hspQK5D	This study	N/A
pET28b- hspQK5E	This study	N/A
pET28b- hspQK5R	This study	N/A
pET28b- hspQD25N	This study	N/A
pET28b- hspQD27N	This study	N/A
pET28b- hspQS93A	This study	N/A
pET28b- hspQRHQ/AAA	This study	N/A
pET28b- hspQext	This study	N/A
pET28b-ymoA	This study	N/A
pET28b-lonYP	This study	N/A
pET28b-lonEC	This study	N/A
2BT- hspQY10	This study	N/A
2BT- hspQY20	This study	N/A
2BT-y2853	This study	N/A
2BT-y2853150	This study	N/A
2BT-y2853DD	This study	N/A
pDEST17- rsuA	This study	N/A
pDEST17- crp	This study	N/A
pDEST17- y0390	This study	N/A
pDEST17- fur	This study	N/A
2BT-cp60Y20	This study	N/A
2BT-cp6-Sul20	This study	N/A
Software and Algorithms
GraphPad Prism for data organization, analysis, and presentation	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Imagestudio™ for densitometry	LI-COR Biosciences	https://www.licor.com/bio/products/software/image_studio/
Canvas™ Draw 3 for Mac for creation of technical illustrations	ACD Systems	http://www.canvasgfx.com/en/products/canvasdraw