Table 1.
Key resources.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Monoclonal Anti-ß-Actin antibody produced in mouse ((1:10000)) | Sigma | Cat#A2228 |
| GAPDH (14C10) Rabbit mAb (1:5000) | Cell Signaling | Cat#2118 S |
| Anti-AMPK alpha 1 antibody (1:1000) | Abcam | Cat#ab3759 |
| Anti-AMPK alpha 2 antibody (1:1000) | Abcam | Cat#ab3760 |
| AMPKa1 Antibody (1:500, IHC) | Cell Signaling | Cat#2795 S |
| AMPKa2 Antibody (1:250, IHC) | Cell Signaling | Cat#2757 S |
| Human/Mouse/Rat AMPK alpha 1 Affinity Purified Pab (Goat) (7.5 μg/mL) | R&D Systems | Cat#AF3197 |
| PSD95 (D27E11) XP® Rabbit mAb (1:1000) | Cell Signaling | Cat#3450 P; RRID:AB_10828082 |
| mTOR (7C10) Rabbit mAb (1:1000) | Cell Signaling | Cat#2983 S; RRID:AB_2105622 |
| Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (1:1000) | Cell Signaling | Cat#5536 S; RRID:AB_10691552 |
| Phospho-mTOR (Ser2481) Antibody (1:1000) | Cell Signaling | Cat#2974 S; RRID:AB_2231885 |
| p70 S6 Kinase (49D7) Rabbit mAb (1:1000) | Cell Signaling | Cat#2708 S; RRID:AB_390722 |
| Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb (1:1000) | Cell Signaling | Cat#9234 S; RRID:AB_2269803 |
| Phospho-p70 S6 Kinase (Ser371) Antibody, Rabbit (1:1000) | Cell Signaling | Cat#9208 S; RRID:AB_330990 |
| Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody (1:1000) | Cell Signaling | Cat#9204 S; RRID:AB_2265916 |
| 4E-BP1 (53H11) Rabbit mAb (1:1000) | Cell Signaling | Cat#9644 S; RRID:AB_2097841 |
| Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (1:1000) | Cell Signaling | Cat#2855 S; RRID:AB_560835 |
| Akt (pan) (C67E7) Rabbit mAb (1:1000) | Cell Signaling | Cat#4691 S; RRID:AB_915783 |
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (1:2000) | Cell Signaling | Cat#4060 S; RRID:AB_2315049 |
| eEF2 Antibody (1:1000) | Cell Signaling | Cat#2332 S; RRID:AB_10693546 |
| Phospho-eEF2 (Thr56) Antibody (1:1000) | Cell Signaling | Cat#2331 S; RRID:AB_10015204 |
| eIF2α Antibody (1:1000) | Cell Signaling | Cat#9722 S; RRID:AB_2230924 |
| Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb(1:1000) | Cell Signaling | Cat#3398 S; RRID:AB_2096481 |
| Phospho-PERK (Thr980) (16F8) Rabbit mAb (1:1000) | Cell Signaling | Cat#3179 S;RRID:AB_2095853 |
| PERK Antibody (H-300) (1:200) | Santa cruz | sc-13073;RRID:AB_2230863 |
| GSK-3β (D5C5Z) XP® Rabbit mAb (1:1000) | Cell Signaling | Cat#12456 S |
| Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (1:1000) | Cell Signaling | Cat#5558 S; RRID:AB_10013750 |
| CaMKII (pan) Antibody (1:1000) | Cell Signaling | Cat#3362 S;RRID:AB_10692639 |
| Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb (1:1000) | Cell Signaling | Cat#12716; RRID:AB_2713889 |
| PKM ζ Antibody (H-1) (1:10000) | Santa Cruz | Cat#sc-17781; RRID:AB_628148 |
| Anti-Kv4.2 (1:1000) | Alomone Labs | Cat#APC-023; RRID:AB_2040176 |
| p-Kv4.2 Antibody (C-6) (1:300) | Santa Cruz | Cat#sc-377574 |
| AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb (1:1000) | Cell Signaling | Cat#13185 S |
| ATF4 (D4B8) Antibody (1:1000) | Cell Signaling | Cat#11815 S |
| Oligophrenin-1 Antibody (1:1000) | Cell Signaling | Cat#11939 S |
| Anti-Puromycin Antibody, clone 12D10 (1:5000) | EMD Millipore | MABE343 |
| Goat Anti-Mouse IgG (H + L)-HRP Conjugate (1:5000) | Bio-Rad | Cat#170-6516; RRID:AB_11125547 |
| Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:5000) | Bio-Rad | Cat#170-6515; RRID:AB_11125142 |
| Goat anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor® 488 conjugate (1:250) | Life Technology | Cat#A-11008; RRID: AB_143165 |
| Donkey anti-Goat IgG (H + L) Secondary Antibody, Alexa Fluor® 633 conjugate (1:250) | Life Technology | Cat#A-21082; RRID:AB_2535739 |
| Biotinylated Goat Anti-Rabbit IgG Antibody | Vector Laboratories | Cat#BA-1000; RRID:AB_2313606 |
| Biotinylated Horse Anti-Goat IgG Antibody | Vector Laboratories | Cat#BA-9500; RRID:AB_2336123 |
| VECTASHIELD Antifade Mounting Medium with DAPI | Vector Laboratories | Cat#H-1200 |
| Bacterial and Virus Strains | N/A | |
| Biological Samples | ||
| Human hippocampal, cerebellum brain blocks | University of Washington Brain Bank | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Pierce™ T-PER™ Tissue Protein Extraction Reagent | Thermo Fisher | Cat#78510 |
| Halt™ Protease Inhibitor Cocktails (with EDTA) | Pierce | Cat#87786 |
| Halt™ Phosphatase Inhibitor Cocktail | Pierce | Cat#78420 |
| Restore™ Western Blot Stripping Buffer | Pierce | Cat#PI21059 |
| SuperBlock (TBS) Blocking Buffer | Pierce | Cat#PI37581 |
| 2-Mercaptoethanol (Reagent), Fisher Chemical | Thermo Fisher | Cat#O3446I-100 |
| SYBR Safe DNA Gel Satin 10,000× | Invitrogen | Cat#S33102 |
| PERK Inhibitor I, GSK2606414 - CAS 1337531-89-1 - Calbiochem | Millipore | Cat#516535 |
| Critical Commercial Assays | ||
| FD Rapid GolgiStain™ Kit | FD NeuroTechnologies, Inc. | Cat#PK401 |
| Deposited data | ||
| Experimental Models: Cell Lines | N/A | |
| Experimental Models: Organisms/Strains | ||
| Mouse:B6.Cg-Tg(Camk2a-cre)T29-1Stl/J | The Jackson Laboratory | Stock No: 005359 | T29-1 |
| Mouse:STOCK Prkaa1tm1.1Sjm/J | The Jackson Laboratory | Stock No: 014141 | Prkaa1 fl |
| Mouse:STOCK Prkaa2tm1.1Sjm/J | The Jackson Laboratory | Stock No: 014142 | Prkaa2fl |
| Oligonucleotides | ||
| Primer: Camk2a-Cre-oIMR1084 Forward: GCG GTC TGG CAG TAA AAA CTA TC | The Jackson Laboratory | Stock No: 005359 | T29-1 |
| Primer: Camk2a-Cre-oIMR1085 Reverse: GTG AAA CAG CAT TGC TGT CAC TT | The Jackson Laboratory | Stock No: 005359 | T29-1 |
| Primer: Prkaa1-11528 CCC ACC ATC ACT CCA TCT CT | The Jackson Laboratory | Stock No: 014141 | Prkaa1 fl |
| Primer: Prkaa1-11529 AGC CTG CTT GGC ACA CTT AT | The Jackson Laboratory | Stock No: 014141 | Prkaa1 fl |
| Primer: Prkaa2-11530 GCA GGC GAA TTT CTG AGT TC | The Jackson Laboratory | Stock No: 014141 | Prkaa1 fl |
| Primer: Prkaa2-11531 TCC CCT TGA ACA AGC ATA CC | The Jackson Laboratory | Stock No: 014141 | Prkaa1 fl |
| Recombinant DNA | N/A | |
| Software and algorithms | ||
| ImageJ (Version1.48) | NIH | https://imagej.nih.gov/ij/ |
| GraphPad Prism (Version 6.05) | GraphPad Software Inc | http://www.graphpad.com/scientific-software/prism/ |
| EthoVision XT (Version 7) | Noldus | http://www.noldus.com/EthoVision-XT/more-about-ethovision-xt |