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. 2021 Apr 19;18:94. doi: 10.1186/s12974-021-02147-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6. — Human Huntington’s disease PSC-derived microglia conditioned media are not toxic to human PSC-derived striatal neurons. IsoHD PSC-derived striatal neurons were cultured with supernatants harvested from IsoHD PSC-derived microglia. a, b Numbers of recipient cells demonstrating caspase 3 activation as a marker of apoptosis were not affected by exposure for five days to conditioned media from microglia expressing mutant HTT, when either a the microglia were unstimulated or b the microglia had been activated by treatment with 1 μg/ml LPS and 10 ng/ml IFNγ for 24 h. c, d Numbers of recipient cells showing increased numbers of γH2ax foci, a marker of DNA damage and genotoxic stress, were lower in HTT 45^Q and 81^Q neurons than 30^Q ones, irrespective of whether they had been treated or not with conditioned media from either c unstimulated or d LPS-stimulated mutant HTT-expressing microglia. Data are presented as mean ± SEM, n = 3, analysed by two-way ANOVA with Tukey’s multiple comparisons test