. 2021 Apr 19;12:53. doi: 10.1186/s40104-021-00572-4

Table 5.

The contents of ROS, MDA, PC, and GSH, and the activities of ASA, AHR, T-SOD, CAT, GPX, GST, and GR in the muscle of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks

Items	Dietary Ile level, g/kg							SEM	P-values
Items	5.0	7.5	10.0	12.5	15.0	17.5	20.0	SEM	Linear	Quadratic
ROS, % DCF fluorescence	100.00^de	81.84^c	61.68^b	37.79^a	36.13^a	86.07^cd	113.90^e	0.72	< 0.0001	< 0.0001
MDA, nmol/mg protein	3.31^b	2.29^ab	1.65^a	1.56^a	1.98^a	2.50^ab	5.47^c	0.27	< 0.01	< 0.0001
PC, nmol/mg protein	6.53^c	6.06^bc	4.40^ab	3.60^a	4.05^a	4.52^ab	5.01^abc	0.31	< 0.0001	< 0.0001
GSH, mg/g protein	2.07^a	2.76^bc	3.25^cd	3.51^d	3.11^cd	2.86^bcd	2.37^ab	0.12	0.15	< 0.0001
ASA, U/mg protein	143.59^a	181.50^b	240.67^cd	284.19^e	245.60^d	209.21^bc	176.86^ab	4.19	< 0.0001	< 0.0001
AHR, U/mg protein	93.45^a	95.32^a	184.63^e	163.67^d	146.11^c	114.58^b	102.72^ab	2.06	< 0.05	< 0.0001
T-SOD, U/mg protein	39.35^ab	41.11^ab	43.89^b	46.63^b	41.34^ab	39.27^ab	34.11^a	1.23	< 0.01	< 0.0001
CAT, U/mg protein	1.47^a	2.49^b	3.33^c	3.21^c	3.15^bc	2.99^bc	2.94^bc	0.15	< 0.0001	< 0.0001
GPX, U/mg protein	34.08	36.12	38.32	40.19	43.72	41.62	40.31	1.84	< 0.01	0.06
GST, U/mg protein	96.58^bcd	100.03^bcd	108.74^d	105.23^cd	93.68^abc	89.56^ab	82.48^a	1.81	< 0.0001	< 0.0001
GR, U/mg protein	5.21^a	6.94^bc	8.02^cd	9.76^e	9.86^e	9.55^de	5.62^ab	0.27	< 0.0001	< 0.0001

Values are mean ± SEM of three replicate groups with 6 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)