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. Author manuscript; available in PMC: 2021 Apr 19.
Published in final edited form as: Prog Biophys Mol Biol. 2019 Mar 21;147:33–46. doi: 10.1016/j.pbiomolbio.2019.03.005

Table 1.

Red-related SynExo proteins that have been characterized biochemically and/or tested for recombineering activity in either E. coli or their native or closely related bacterial host.a

Bacterial host Phage (or prophage) Exo/SSAP Accessory Reference
S. enterica phage P22 Abc2b-RecBCD/Erfb Abc2, Arf Poteete & Fenton (1993)
E. coli Rac prophage RecEb/RecTb Kolodner et al. (1994)
E. coli phage λ λ exob/Redβb gam, rap, orf Yu et al. (2000)
B. subtilisd phage SPP1 G34.1Pb/G35Pb G36P Martínez-Jiménez et al. (2005); Datta et al. (2008)
M. smegmatis phage Che9c gp61b/g60b van Kessel and Hatfull (2007); Datta et al. (2008)
E. faecalisc prophage EF2131/EF2132 Datta et al. (2008)
L. pneumophilad prophage orfB/orfC Datta et al. (2008)
V. choleraec SXT (ICE)e s066b/s065 Datta et al. (2008); Chen et al. (2011)
P. luminescensd prophage Plu2936/Plu2935 Plu2934 Datta et al. (2008); Yin et al. (2015)
L. monocytogenesd phage A118 orf47/orf48 Datta et al. (2008)
L. lactisc phage ul36.2 ———/Orf245 Datta et al. (2008)
S. aureusc phage phiNM3 ——/GP20 Datta et al. (2008)
P. syringae prophage RecEPsy/RecTPsy Swingle et al. (2010)
L. honkogenesis prophage LHK-Exob/LHK-Bet Yang et al. (2011)
L. reuterif,g prophage —/RecT1 van Pijkeren & Britton (2012)
C. perfringensg,h prophage —/CPF0939 Dong et al. (2014)
P. aeruginosa D3 phage exo/erf De Paepe et al. (2014)
L. planatarum P1 prophage lp_0642/lp_0641 lp_0640 Yang et al. (2015)
P. putidae prophage T1E1404/T1E1405 Aparicio et al. (2016)
a

Similar tables have been provided in Datta et al. (2008), and Yin et al. (2015).

b

These proteins have been purified and characterized for DNA binding and nuclease activities in vitro.

c

These systems were tested in E. coli for ssDNA recombineering.

d

These systems were tested in E. coli for ssDNA and dsDNA recombineering.

e

ICE: integrating conjugative element (a mobile genetic element).

f

The RecT1 protein from L. reuiteri was also tested in L. lactis, L. gasseri, and L. plantarum.

g

For these SSAPs only ssDNA recombineering was tested.

h

The recT homolog from C. perfringens was tested for ssDNA recombineering in C. acetobutylicum.