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. 2021 Mar 30;65(2):3226. doi: 10.4081/ejh.2021.3226

Figure 3.

Figure 3.

Cell proliferation assay in MYBL2 knockdown EC cell lines. A) The capability of different shRNAs to downregulate MYBL2 expression. B) The ability of different shRNAs to inhibit the expression of MYBL2 protein. C) The cell proliferation rate in HEC-1-B and AN3CA cells at 48 h after MYBL2 knockdown; after transfecting the two cell lines with MYBL2 shRNA, MYBL2 expression was downregulated, and the cell proliferation of HEC-1-B and AN3CA cells was inhibited dramatically compared to that of the sh-NC cells. D) CCK-8 cell growth curve. E) Knockdown of MYBL2 significantly reduced the colony forming ability of HEC-1-B and AN3CA cell lines. **p<0.01; ***p<0.001.