Figure 1. Determination of the genotypes of the -174 G/C polymorphism in IL6 gene. Left: Agarose gels with homozygous GG (140 and 58 bp) and heterozygous GC (198, 140 and 58 bp) genotypes as determined by PCR-RFLP with SfaNI as the restriction enzyme 32. Right: Chromatograms obtained by automated Sanger sequencing confirming the GG and CC genotypes (marked with arrows).
