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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2020 Dec 11;1868(3):118929. doi: 10.1016/j.bbamcr.2020.118929

Figure 6: TBK1 inhibition and depletion results in mitotic aberrations.

Figure 6:

TBK1 was either chemically inhibited or depleted using CRISPR/ Cas9 techniques in A549 (A), H460 (B), MDA-MB-231 (C) and Hs578T (D) cells; they were then stained for α tubulin (green) and γ tubulin (red) to study mitotic aberrations, specifically centrosomal amplification. (E) MDA-MB-231 cells were treated with 2.5μM BX795 for TBK1 inhibition and stained with α Tubulin and DAPI show multinucleation. (F) Micronucleation was studied in MDA-MB-231 cells after knockdown of TBK1 or TTK using siRNA transfection. A total of 200 cells were considered and micronuclei counted for each condition for quantification. Plot represents mean of three independent experiments. A one-way ANOVA-test was done for significance. p≤ 0.05 was considered significant. Error bars are mean ± S.D.