Fig. 4.

Low-grade PRB-H human samples express higher levels of nuclear HMW-FGF2 than low-grade PRA-H samples. a FGF2 immunohistochemistry. Representative images of cytosolic (left) and nuclear (right) FGF2 expression in a subset of luminal breast cancer samples. Bar 50 μm. b Percentage of PRA-H or PRB-H samples with a FGF2 nuclear score > 90. c Western blot analysis and d quantification of FGF2 expression in nuclear cell extracts from human breast cancer samples. Total ERK was used as loading control. FGF2 expression was normalized to a positive control which was run in all gel electrophoresis. nFGF2, nuclear FGF2; HMW, high molecular weight; LMW, low molecular weight