Eight-week-old male rats were treated with loperamide along with 3.2 × 1010, 8 × 1010, and 1.6 × 1011 cells/mLHLp-nF1, and Dulcolax, individually. A single treatment with 1.6 × 1011 cells/mLHLp-nF1 or Dulcolax was used as the control group. The intestinal transit length (A) and intestinal transit percentage (B) were measured and quantified as described in the Materials and Methods. The quantification analysis is shown. Data represent the mean ± SD. A t-test was used to determine the means of two groups (n = 7). *P< 0.05 vs. control group, #P< 0.05 vs. loperamide-treated group. Con, control group; Lop, loperamide-treated group; Dul, Dulcolax-treated group (0.75 mg/kg); HHL, treatment with 1.6 × 1011 cells/mL HLp-nF1; Lop+LHL, treatment with loperamide and 3.2 × 1010 cells/mL HLp-nF1; Lop+MHL, treatment with loperamide and 8 × 1010 cells/mL HLp-nF1; Lop+HHL, treatment with loperamide and 1.6 × 1011 cells/mL HLp-nF1; Lop+Dul treated group.