Fig 5. Effect of HLp-nF1 on intestinal contractility.

Eight-week-old male rats were treated with loperamide with or without 8 × 10¹⁰ cells/mL HLp-nF1 and Dulcolax individually. (A) After isolation from the treated rats, the ileum was treated with 1 μM acetylcholine, and then with 1 μM phenylephrine. (B) Quantification of acetylcholine-induced intestine contraction. (C) Quantification of phenylephrine-induced intestine relaxation and recovery. A t-test was used to determine the means of two groups (n = 4). *P< 0.05 vs. control group, ^#P< 0.05 vs. loperamide-treated group). Con, control group; Lop, loperamide-treated group; Lop+MHL, treatment with loperamide and 8 × 10¹⁰ cells/mL HLp-nF1; Lop+Dul treated group.