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A. 1PGE-THIVC SOSIP was first purified by PGT145 antibody affinity column followed by size exclusion chromatography (SEC). B. The purified SOSIP trimer was examined by blue native gel electrophoresis. C. Efficient cleavage of 1PGE-THIVC trimeric Env was examined by running in SDS-PAGE under reducing conditions. D. The physical conformation of the SEC purified SOSIP trimers were examined by two-dimensional negative-stain EM class averages (D).
