Table 3. Mapping fine specificity of rabbit serum antibodies associated with neutralization of autologous virus.
| Fold change in neutralization of Env-pseudotyped viruses | |||
|---|---|---|---|
| Env constructs | ID50 values | Fold increase | Fold decrease |
| PG80v1.eJ7 wild type | 1798.6 | - | - |
| PG80v1.eJ19 wild type | 2117 | - | - |
| PG80v2.eJ38 wild type | 97.58 | - | - |
| PG80v2.eJ38 (V1/V2 of v1.eJ7) | <20 | No change | No change |
| PG80v2.eJ38 (V3/C3 of v1.eJ7) | <20 | No change | No change |
| PG80v2.eJ38 (V3/C4 of v1.eJ7) | 664.18 | 6.8 | - |
| PG80v2.eJ38 (C4/C5 of v1.eJ7) | 53.47 | No change | - |
| PG80v2.eJ38 (K360R) | 102.28 | No change | - |
| PG80v2.eJ38 (L401P) | 418.46 | 4.3 | - |
| PG80v2.eJ38 (N362T+K360R | 181 | 1.85 | - |
| PG80v2.eJ38 (N362T+L401P) | 789.68 | 8.1 | - |
| PG80v1.eJ7 (T362N+P401L) | 101.54 | - | 17 |
| PG80v1.eJ19 (T362N+P401L) | 171 | - | 12.4 |
| PG80v1.eJ19 (T362N) | 400.72 | - | 5.28 |
| PG80v1.eJ19 (P401L) | 337.96 | - | 6.26 |
ID50 values refer to reciprocal dilution that conferred 50% neutralization of Env-pseudotyped viruses in TM-bl cells. Neutralization assays were done in triplicates and repeated at least two times.