Figure 7.

Association of the catalytic protein kinase A (PKA) subunit with proteasome during human sperm capacitation. (A) Co-immunoprecipitation (IPP) of the proteasome and the catalytic subunit of PKA. Spermatozoa were incubated for 0 or 15 min. After incubation, the spermatozoa were lysed in lysis buffer and immunoprecipitated overnight with an anti-PSMA7 proteasome subunit antibody. The PKA catalytic subunit was detected by western blotting (WB). Cell extracts correspond to the lysates of individual treatment groups. (B) For densitometry, the α4 subunit was used as a control. Bars represent the mean ± SEM of three different experiments. Different letters indicate statistically significant differences (P < 0.01) between the groups.