Figure 3.

Downstream targets of REST are dysregulated in SNCA-OVX mice and reside in distinct regions of the midbrain. a, Protein-protein interaction network of upstream regulators in SNCA-OVX mice (p < 0.05). b, Directed REST interaction network showing the upregulation and downregulation of REST target proteins in SNCA-OVX mice; downregulated (blue) and upregulated (red) proteins and predicted effect of REST on downstream targets (arrows). Green arrows indicate protein regulation consistent with REST inhibition. Orange arrows indicate protein level changes inconsistent with REST inhibition. Black lines indicate proteins without directionality assigned. c, d, Immunofluorescence of mouse midbrain demonstrating (c) location of TH (green) in the SNc and NEFH (red) in the SNr and (d) colabeling of NEFH (green) and parvalbumin (PV; red) cells in 3-month-old WT (or SNCA-OVX) mouse midbrain.