Table 5.
Quench solution-II.
| Component | Volume (mL) |
Final concentration (mM) |
|---|---|---|
| NF H2O | 4.9 | |
| 0.5 M EDTA, adjust to pH 8.0 with 5 M NaOH | 4 | 200 |
| 0.4 M NEM | 1 | 40 |
| 0.2 M NaAsO2 | 0.1 | 2 |
| Final Volume | 10 |
Quench solution-II.
| Component | Volume (mL) |
Final concentration (mM) |
|---|---|---|
| NF H2O | 4.9 | |
| 0.5 M EDTA, adjust to pH 8.0 with 5 M NaOH | 4 | 200 |
| 0.4 M NEM | 1 | 40 |
| 0.2 M NaAsO2 | 0.1 | 2 |
| Final Volume | 10 |