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. Author manuscript; available in PMC: 2022 Feb 12.
Published in final edited form as: Methods Enzymol. 2021 Feb 12;649:371–396. doi: 10.1016/bs.mie.2021.01.023

Table 5.

Quench solution-II.

Component Volume
(mL)
Final concentration
(mM)
NF H2O   4.9
0.5 M EDTA, adjust to pH 8.0 with 5 M NaOH   4 200
0.4 M NEM   1 40
0.2 M NaAsO2   0.1   2
Final Volume 10