Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Dcst1/2 KO | This article | Deposited in RIKEN BRC (ID RBRC05733) | |
| Strain, strain background (Mus musculus) | Dcst1 KO | This article | Deposited in RIKEN BRC (ID RBRC10275) | |
| Strain, strain background (Mus musculus) | Dcst2 KO | This article | Deposited in RIKEN BRC (ID RBRC10366) | |
| Strain, strain background (Mus musculus) | Spaca6 KO | This article | Deposited in RIKEN BRC (ID RBRC10367) | |
| Strain, strain background (Mus musculus) | Izumo1 KO | Inoue et al., 2005 | RRID MGI_3576518 | |
| Strain, strain background (Mus musculus) | Dcst1 TG | This article | Deposited in RIKEN BRC (ID RBRC05856) | |
| Strain, strain background (Mus musculus) | Dcst2 TG | This article | ||
| Strain, strain background (Mus musculus) | Izumo1 TG | Inoue et al., 2005 | ||
| Antibody | Anti-mouse SPACA6 (rabbit polyclonal) | This article | (WB: 1:500) | |
| Antibody | Anti-mouse IZUMO1 (rat monoclonal) | Inoue et al., 2013 | Mab125 | Gift from Dr. Ikawa (IF: 0.5 µg ml−1) |
| Antibody | Anti-mouse IZUMO1 (rat monoclonal) | Inoue et al., 2015 | Mab18 | (WB: 1 µg ml−1) |
| Antibody | Anti-mouse FR4 (JUNO) (rat monoclonal) | BioLegend | TH6, RRID AB_1027724 | (IF: 0.25 µg ml−1) |
| Antibody | Anti-mouse CD9 (rat monoclonal) | BioLegend | MZ3, RRID AB_1279321 | (IF: 0.5 µg ml−1) |
| Antibody | Anti-mouse EMMPRIN (BASIGIN) (goat polyclonal) | Santa Cruz Biotechnology | G-19, RRID AB_2066959 | (WB: 0.2 µg ml−1) |
| Recombinant DNA reagent | Plasmid pX330 vector | Addgene | RRID Addgene_42230 | |
| Sequence-based reagent | Primers | This article | Detailed in 'Materials and methods' | |
| Peptide, recombinant protein | SPACA646-139 | This article | Detailed in 'Materials and methods' |