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. Author manuscript; available in PMC: 2021 Apr 19.
Published in final edited form as: Cell Rep. 2021 Apr 6;35(1):108962. doi: 10.1016/j.celrep.2021.108962

Figure 4. Protection by non-neutralizing mAbs is Fc dependent.

Figure 4.

(A) Four-week-old C57BL/6J FcγR−/− male and female mice were administered 100 μg of MAY-10 or MAY-108 1 day before subcutaneous inoculation of MAYV-BeH407 (two experiments, n = 6).

(B) Isotype-switched mAb binding to recombinant murine FcγRI, FcγRIII, and FcγRIV. MAY-10 or MAY-108 of the indicated isotype were added to plates coated with FcγRs. Binding data: two-way ANOVA with Sidak’s post-test, compared to mIgG2c isotype mAb.

(C–F) MAY-10 (C) and MAY-108 (E) were isotype switched from murine IgG2c to murine IgG1, human IgG1, or human IgG1-N297Q. Each antibody variant was tested for binding to captured MAYV by ELISA. For protection studies (D and F), 100 μg of the indicated mAb was administered to 4-week-old C57BL/6J mice 1 day before subcutaneous inoculation with MAYV (two experiments, n = 6; log-rank test with Bonferroni correction compared to isotype control mAb). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.