Figure 4.

SXSM reduces Ang II-induced ROS accumulation in HL-1 cells. (a) A CCK-8 cell viability assay was used to determine the optimal concentration of SXSM. (b) ROS accumulation in HL-1 cells was assayed using green-fluorescent DCFH-DA. (c) ROS fluorescence photos statistical analysis. CON: control group cells; Ang II: cells treat with Ang II (1 μM); SXSM: cells treated with Ang II and SXSM (1 ml/L); SXSM + PMA: cells treat with Ang II, SXSM (1 mL/L) and the PKC activator PMA (1 μM); ^∗∗p < 0.01. SAN, sinoatrial node; SXSM, Shenxian-shengmai oral liquid; SSS, sick sinus syndrome, Ang II, angiotensin II; ROS, reactive oxygen species.