Table 3S. Repeatability of qPCR assay for defensin A gene in Escherichia coli-infected Aedes aegypti mosquitoes.
Sample | Replicated runs (Ct) | Mean | SD | Repeatability (CV) |
% CV |
||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | |||||
Calibrator | 27 | 27.2 | 26.8 | 27.0 | 0.2 | 0.01 | 0.7 |
5 h | 25.4 | 25.7 | 25.4 | 25.5 | 0.2 | 0.01 | 0.7 |
24 h | 27.9 | 27.1 | 27.5 | 27.5 | 0.4 | 0.01 | 1.5 |
10 d | 25.9 | 26.9 | 26.6 | 26.5 | 0.5 | 0.02 | 1.9 |
15 d | 22.8 | 23.1 | 22.8 | 22.9 | 0.2 | 0.01 | 0.8 |
SD: standard deviation; CV: coefficient of variance