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. 2020 Dec 15;203(4):1299–1308. doi: 10.1007/s00203-020-02125-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Metabolic activity of I. hospitalis (three parallels: IH1, IH2, and IH3) after γ-ray exposure monitored on lead acetate paper. The metabolical produced H₂S reacts with lead acetate forming lead sulfide, visible as dark-brown spots. Flash γ-radiation-exposed cultures, M unexposed ½ SME + S⁰ medium incubated at 90 °C for up to 6 days, DLR laboratory control, BGS transport control. Serial dilutions with 1:10 dilution steps (10^–1–10^–8) are represented by the exponent (Koschnitzki 2016)