Fig. 5.

Effect of dexamethasone on phosphate and Ca²⁺ metabolism. Arithmetic means ± SEM of creatinine clearance (a, n = 6), the serum concentration of phosphate (b, n = 17; 12 h after injection) and Ca²⁺ (c, n = 12; 12 h after injection), as well as phosphate (d, n = 6) and Ca²⁺ (e, n = 6) excretion in mice in 24 h urine after injection of vehicle (ctr, white bars) or 20 mg/kg body weight dexamethasone (DEXA, black bars). Arithmetic means ± SEM (n = 6, arbitrary units (a. u.)) of relative mRNA transcript levels of Slc34a1 (f) and Kl (g) normalized to Gapdh in kidneys from mice 12 h after the injection of vehicle (ctr, white bars) or 20 mg/kg body weight dexamethasone (DEXA, black bars). *p < 0.05, ***p < 0.001 indicate statistically significant differences from control mice. (a, b, d, e and g: unpaired t test; c: Mann-Whitney U test; f: unpaired t test with Welch’s correction)