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. 2021 Apr 12;13:3155–3169. doi: 10.2147/CMAR.S292461

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© 2021 Huang et al.

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The expression of Nrf2, HO-1, and VEGF, and microvessel density increased in tumor-bearing mice. (A–D) Immunocytochemistry was used to determine the expression of Nrf2, HO-1, and VEGF in tumor and normal tissues of tumor-bearing and tumor-free mice, respectively. (E and F) Western blotting was utilized to detect the expression of Nrf2, HO-1, and VEGF in tumor and normal tissues of tumor-bearing and tumor-free mice, respectively. (G and H) Immunohistological staining of CD34 was used to determine the microvessel density in tumor and normal tissues of tumor-bearing and tumor-free mice, respectively.

Notes: Scale bar, 50 μm. **P<0.01.