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. 2021 Apr 19;7:85. doi: 10.1038/s41420-021-00426-y

Fig. 2. JAC1 downregulates HER2 expression and inhibits cell proliferation in breast cancer.

Fig. 2

a Flow chart of a screening strategy for small molecule compounds JWA gene agonist. Through a series of high-throughput screening analyses of 40,000 compounds in the national compound library (Shanghai, China), 650 compounds were obtained. At last, six compounds were identified to have the ability to activate JWA expression according to their molecular weights, lipid water partition coefficients, and chemical structures. b Protein expressions of the HER2 and JWA were determined by western blot. c JWA/wild-type or JWA/KO cells were treated with JAC1 (10 μM) for 24 h. HER2 and JWA protein expressions were determined by western blot. d, e Representative images of the colony formation assay for BT474 and SKBR3 cells after treatment with different concentrations of JAC1 (0, 1, 10 μM). f BT474 and SKBR3 cells were treated with JAC1 (0, 1, 10 μM) for 24 h, immunofluorescence imaging showed expressions of JWA (green), HER2 (red), nucleus (blue), the co-localization of the three signals (merge).