Trypsin solution (store at −80°C)
| Reagent | Final concentration | Amount |
|---|---|---|
| Trypsin | 0.25 μg/μL | 20 μg |
| CaCl2 (100 mM) | 25 mM | 20 μL |
| Trypsin buffer | n/a | 60 μL |
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