Figure 4.
Immunofluorescence of the developing and late organoids
Representative confocal images of retinal organoids at different differentiation days.
(A) Retinal organoids stained with PAX6 (green), CRX (red), Recoverin (green), and DAPI (blue). Scale bars, 50 μm.
(B) Retinal organoids of different groups stained with Rhodopsin (red), L/M-opsin (green), ARL13B (green), and DAPI (blue). Scale bars, 20 μm (top) and 5 μm (bottom). W6, differentiation week 6. W9, differentiation week 9. W12, differentiation week 12. W22, differentiation week 22.
