Differentiation medium:
| Reagent | Final concentration | Amount |
|---|---|---|
| IMDM | 44% | 22 mL |
| F12 | 44% | 22 mL |
| KSR | 10% | 5 mL |
| Glutamax | 1% | 500 μL |
| Monothioglycerol | 450 μM | 1.95 μL |
| PS | 1% (100 U/mL) | 500 μL |
| Total | n/a | 50 mL |
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