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. 2021 Apr 8;2(2):100438. doi: 10.1016/j.xpro.2021.100438

Cell dissociation solution:

Reagent Final concentration Amount
TrypLE Select n/a 1 mL
DNase I (5 mg/mL) 0.05 mg/mL 10 μL
Y-27632 (10 mM) 20 μM 2 μL
Total n/a 1.012 mL