Cell dissociation solution:
| Reagent | Final concentration | Amount |
|---|---|---|
| TrypLE Select | n/a | 1 mL |
| DNase I (5 mg/mL) | 0.05 mg/mL | 10 μL |
| Y-27632 (10 mM) | 20 μM | 2 μL |
| Total | n/a | 1.012 mL |
Cell dissociation solution:
| Reagent | Final concentration | Amount |
|---|---|---|
| TrypLE Select | n/a | 1 mL |
| DNase I (5 mg/mL) | 0.05 mg/mL | 10 μL |
| Y-27632 (10 mM) | 20 μM | 2 μL |
| Total | n/a | 1.012 mL |