Fig. 2.
Global deletion of Tlr8 or knockdown of Tlr8 in the TG alleviates the mechanical allodynia induced by pIONL. A Nocifensive behavior scores stimulated by a 0.02 g von Frey filament in WT and Tlr8−/− mice (**P < 0.01, ***P < 0.001 vs WT, two-way RM ANOVA followed by the Bonferroni test). B Nocifensive behavior scores stimulated by a 0.02 g von Frey filament after intra-infraorbital nerve injection of Tlr8 siRNA mixed with RVG 4 days after pIONL (***P < 0.001 vs NC siRNA, two-way RM ANOVA followed by the Bonferroni test). C RT-PCR of TG tissue showing that Tlr8 siRNA down-regulates Tlr8 mRNA expression (*P < 0.05 vs NC siRNA, Student’s t-test; n = 5 mice/group).