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. 2021 Apr 19;11:57. doi: 10.1186/s13568-021-01218-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Time course of Miller units determined by the Miller Assay representing expression of promoters P_srfA, P_narG and P_nasD. Data recorded until CDW_max of cultivation of strains B. subtilis KM1016 (P_srfA-lacZ), MG1 (P_narG-lacZ) and MG5 (P_nasD-lacZ) in a mineral salt medium employing 40 g/L glucose, 0.1 mol/L NH₄⁺ and 0.1 mol/L NO₃⁻ in baffled shake flasks with three different relative filling volumes (Rv) of 10%, 50% and 100% representing different oxygen availabilities