Table 3.
Strategy | Reference pO2 set-point 20% | Fed-batch, constant aeration of 2 L/min | Fed-batch, constant aeration of 1.2 L/min |
Fed-batch, constant aeration of 1.2 L/min |
|||
---|---|---|---|---|---|---|---|
Evaluation until | End of batch | After batch glucose consumption | End of process | After batch glucose consumption | End of process | After batch glucose consumption | End of process |
B. subtilis strain | JABs24 | MG1 | KM1016 | MG14 | |||
Time of cultivation (h) | 24 | 22 | 40 | 26 | 46 | 19 | 46 |
CDWmax (g) | 77.51 | 104.55 | 196.97 | 61.54 ± 9.21 | 147.52 ± 8.22 | 55.78 ± 13.24 | 150.18 ± 3.33 |
Surfactinmax (g) | 57.43 | 51.65 | 104.57 | 48.47 ± 11.81 | 82.14 ± 0.91 | 15.49 ± 6.40 | 31.56 ± 3.74 |
YP/X (g/g) | 0.741 | 0.494 | 0.531 | 0.776 ± 0.088 | 0.557 ± 0.024 | 0.266 ± 0.039 | 0.211 ± 0.030 |
YX/S (g/g) | 0.145 | 0.249 | 0.195 | 0.161 ± 0.011 | 0.131 ± 0.005 | 0.134 ± 0.016 | 0.123 ± 0.003 |
YP/S (g/g) | 0.108 | 0.124 | 0.103 | 0.127 ± 0.022 | 0.073 ± 0.001 | 0.036 ± 0.010 | 0.026 ± 0.004 |
q (g/(g∙h)) | 0.031 | 0.022 | 0.013 | 0.030 ± 0.003 | 0.012 ± 0.001 | 0.014 ± 0.002 | 0.004 ± 0.001 |
µ (1/h) | 0.222 | 0.272 | 0.165 | 0.204 ± 0.009 | 0.134 ± 0.005 | 0.271 ± 0.010 | 0.128 ± 0.003 |