Fig. 4.

Transgenic lines overexpressing SlHSP90.2 expresses more SlHSP90.2 than control plants. a A schematic representation of pCAMBIA2301 transformation vector showing SlHSP90.2 and GUS (β-glucuronidase) reporter genes under the control of 35S promoter (35SP) and NosA terminator. b Analysis of GUS expression in the roots of three independent transgenic lines. Untransformed Pusa Ruby and an empty vector transformed transgenic line were used as controls. c Relative expression of SlHSP90.2 in the 35S::SlHSP90.2 transgenic lines was analyzed using qRT-PCR. The data indicate means ± SD from three independent biological replicates each with three technical replicates. Normalization was done using SlACTIN