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. 2021 Apr 6;15:657445. doi: 10.3389/fncir.2021.657445

FIGURE 1.

FIGURE 1

Characterization of PICs in 5-HT neurons. (A) The transverse slices were collected from the medulla indicated by dash lines. (B) One slice cut from the sections shows the EYFP+ serotonin (5-HT) neurons with enhanced yellow fluorescent proteins in parapyramidal region (PPR) and midline raphe nuclei (MRN) areas. (C) Measurement of biophysical parameters of PICs by the voltage bi-ramp from −70 to 50 mV. (D) Measurement of biophysical parameters (recruitment currents, decruitment currents, and difference ΔI) from repetitive firing induced by current bi-ramp with a duration of 10 s, peak of 60–80 pA, and holding current of zero pA. (E) Distribution of 5-HT neurons with PICs in medulla (top). Proportions of PICs (solid) and Non-PICs (open) recorded from PPR and MRN 5-HT neurons (bottom). (F) Statistic results of the onset and amplitude of PICs in PPR (n = 107) and MRN (n = 91). Error bars show SD, unpaired t-test. (G) Four patterns of PICs in 5-HT neurons of medulla. (G1) a-PIC only with counterclockwise hysteresis. (G2) a-PIC > d-PIC with counterclockwise hysteresis of PICs. (G3) d-PIC only with clockwise hysteresis. (G4) d-PIC > a-PIC with clockwise hysteresis of PICs.