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. 2021 Apr 19;11:8422. doi: 10.1038/s41598-021-87656-3

Figure 3.

Figure 3

Mitochondrial abnormalities in agoTRiPDf testis. (a-b) ATP5alpha with anti-Atp5 (green), nuclei with DAPI (blue), and investment cones with Phalloidin-TexasRed were visualized on control (a) and on agoTRiPDf (b) in early elongating stages. Arrows show mitochondrial bulges next to the elongation complex in agoTRiPDf cyst(b). (c,d) Phase-contrast images of elongating cysts in control (c) and agoTRiPDf (d) testis with vacuolar structures (arrows). (e,f) Anti-ATP5alpha staining on wild type (e) and agoTRiPDf (f) of elongated cysts: staining reveals mitochondrial swelling in agoTRiPDf spermatids. (g,h) Anti-AXO49 (green) staining on control (g) and on agoTRiPDf (h). Cysts from the agoTRiPDf testis elongate normally compared to control (g,h,i). Statistical significance was determined by Welch two-sample t-test (i).( j-m) Transmission electron micrographs of wild type (j), agoTRiPDf (k-m). We can observe multiple mitochondrial abnormalities in agoTRiPDf, like paracrystalline accumulation in both mitochondrial derivatives (k,l,m yellow arrows), mitochondrial swelling (l, red arrows) and misshaped mitochondria (m red arrow) The charts was created in Microsoft Excel 2016 MSO ver. 16.0.4266.1001 and processed in Adobe Illustrator CS6 ver. 16.0.3.