Table 1.

Effect of different concentrations of fungicide mancozeb and compounds isolated from Monotheca buxifolia on growth appearance and biomass of Macrophomina phaseolina.

Methanolic extract fraction	Conc. of DMSO (µL mL−1)	Compound conc (mg mL−1)	Fungal biomass (mg)	Decrease over control (%)
Control	0.104	0.0000	23.4 A	–
	0.208	0.0000	22.6 AB	–
	0.416	0.0000	22.8 AB	–
	0.832	0.0000	21.1 BC	–
	1.66	0.0000	21.3 B	–
	3.33	0.0000	21.0 BC	–
	6.66	0.0000	19.2 C	–
Mencozeb	0.104	0.0312	3.9 Za–e	83
	0.208	0.0625	3.7 a–e	84
	0.416	0.1250	3.8 a–e	83
	0.832	0.2500	3.5 d–e	83
	1.66	0.5000	3.5 b–e	83
	3.33	1.0000	3.3 c–e	85
	6.66	2.0000	3.1 e	84
Lupeol (1)	0.104	0.0312	13.3 DE	43
	0.208	0.0625	13.4 D	41
	0.416	0.1250	13.4 D	41
	0.832	0.2500	12.8 D–F	39
	1.66	0.5000	12.3 D–G	42
	3.33	1.0000	12.5 D–G	40
	6.66	2.0000	11.6 D–H	40
Lupeol acetate (2)	0.104	0.0312	4.6 T-Za–e	80
	0.208	0.0625	4.6 T-Za–e	80
	0.416	0.1250	4.3 V-Za–e	81
	0.832	0.2500	4.3 V-Za–e	80
	1.66	0.5000	4.0 Y-Za–e	81
	3.33	1.0000	3.9 Za–e	81
	6.66	2.0000	4.0 Y–Za–e	79
Betulin (3)	0.104	0.0312	5.7 O–Za	76
	0.208	0.0625	5.3 P–Za–c	77
	0.416	0.1250	5.2 P–Za–d	77
	0.832	0.2500	4.5 U–Za–e	79
	1.66	0.5000	4.7 S–Za–e	78
	3.33	1.0000	4.5 U–Za–e	79
	6.66	2.0000	4.2 X–Za–e	78
β-Sitosterol (4)	0.104	0.0312	9.4 I–K	60
	0.208	0.0625	9.4 I–K	58
	0.416	0.1250	9.0 J–L	61
	0.832	0.2500	9.1 J–L	57
	1.66	0.5000	8.0 K–M	62
	3.33	1.0000	7.7 K–N	64
	6.66	2.0000	7.7 K–N	60
β-Amyrin (5)	0.104	0.0312	7.4 L–O	68
	0.208	0.0625	6.9 M–Q	69
	0.416	0.1250	6.5 M–U	71
	0.832	0.2500	6.6 M–T	69
	1.66	0.5000	6.6 M–T	69
	3.33	1.0000	6.2 M–X	70
	6.66	2.0000	6.2 M–X	68
Oleanolic acid (6)	0.104	0.0312	6.8 M–R	71
	0.208	0.0625	6.7 M–S	70
	0.416	0.1250	6.7 M–S	71
	0.832	0.2500	6.3 M–V	70
	1.66	0.5000	6.5 M–U	69
	3.33	1.0000	6.3 M–V	70
	6.66	2.0000	6.0 N–Y	68
Vanillic acid (7)	0.104	0.0312	6.2 M–X	74
	0.208	0.0625	5.6 O–Za	76
	0.416	0.1250	5.6 O–Za	79
	0.832	0.2500	5.4 O–Zab	74
	1.66	0.5000	4.7 S–Za–e	78
	3.33	1.0000	4.9 R–Za–e	77
	6.66	2.0000	4.9 R–Za–e	74
Protocatechuic acid (8)	0.104	0.0312	6.9 M–Q	71
	0.208	0.0625	6.7 M–S	70
	0.416	0.1250	6.9 M–Q	70
	0.832	0.2500	6.9 M–Q	67
	1.66	0.5000	6.5 M–U	72
	3.33	1.0000	6.2 M–X	70
	6.66	2.0000	5.9 N–Z	69
Kaempferol (9)	0.104	0.0312	12.3 D–G	50
	0.208	0.0625	11.6 D–H	50
	0.416	0.1250	11.3 E–I	50
	0.832	0.2500	11.3 E–I	46
	1.66	0.5000	10.8 G–J	58
	3.33	1.0000	9.0 J–L	57
	6.66	2.0000	9.1 J–L	53
Quercetin (10)	0.104	0.0312	12.0 D–H	49
	0.208	0.0625	12.0 D–H	47
	0.416	0.1250	11.8 D–H	48
	0.832	0.2500	11.2 F–I	47
	1.66	0.5000	10.1 H–J	53
	3.33	1.0000	9.5 I–K	55
	6.66	2.0000	9.0 J–L	53
	HSD0.001		2.01

Values with different letters in a column show significant difference (P ≤ 0.001) as determined by Tukey’s HSD test.