TABLE 6.

A model representing state of art for selecting the genes which can be edited to domesticate crop wild ancestral species through CRISPR/Cas9 approach.

Crop Name	Target Gene	Function	References
Zea Mays	Tb1	TCP-gene family TF which is involved in suppression of side branching changes the source/sink relationships; yields increase.	Doebley et al., 1997; Studer et al., 2011
	tga1	SBP-box TF have a key role in alteration of the encased kernel to naked kernel	Wang et al., 2015
	CCT	CCT domain-containing protein gene involved in decrease of photoperiod sensitivity	Yang et al., 2013; Huang et al., 2018
Glycine max	DT1	CETS is a family of regulatory genes which are involved in transforming indeterminate growth to determinate, resulting in developing a compact crop.	Tian et al., 2010; Cai et al., 2018
	GA20ox	Key enzyme involved in Gibberellin biosynthesis and identified as its association with seed weight	Lu et al., 2016
	SHAT1-5	Plant specific NAC gene family TF involved in the biosynthesis of secondary cell wall which facilitating fiber cell cap thickening result in a decreasing the rate of pod shattering	Dong Y. et al., 2014
Solanum lycopersicum	ARF19	Auxin response factor 19 TF reported being a negative regulator of fruit set	De Jong et al., 2009
	BRC1a	BRANCHED1a gene encoding a TCP family TF which involved in the regulation of lateral shoot outgrowth	Martín-Trillo et al., 2011
	CHI	Chalcone Isomerase is associated with flavonoid biosynthesis	Willits et al., 2005
	S	Compound inflorescence (s) encodes a homeobox TF which controls the number of flower/fruits per inflorescence architecture	Lippman et al., 2008
	CKX	Cytokinin oxidase enzyme associated gene is involved in the inactivation of bioactive cytokinin	Ashikari et al., 2005
	FAS	CLAVATA3 encoded the Fasciated gene which is associated with controlling locules number and size in fruit	Xu C. et al., 2015
	GLK2	Golden2-like TF belongs to GARP family which play a key role in the regulation of chloroplast development in fruits	Powell et al., 2012
	J1	JOINTLESS belongs to MADS-box gene family controlling the development of the abscission zone in pedicels	Mao et al., 2000
	Cyc-B	Lycopene β-cyclase involved in the catalyzes the conversion of lycopene into β-carotene	Ronen et al., 2000
	NOR	Non-ripening gene associated with the initiation of the normal fruit ripening	Seymour et al., 2013
	O	OVATE is a regulatory gene involved in the regulation of fruit shape	Liu et al., 2002
	PRO	PROCERA gene involved in suppression of gibberellin signaling	Jasinski et al., 2008
	RIN	RIPENING INHIBITOR gene belongs MADS-box family; key role in controlling biosynthesis of ripening -related ethylene	Seymour et al., 2013
	SP	SELF-PRUNING gene is a developmental regulator associated with indeterminate and sympodial growth habit in tomato	Pnueli et al., 1998
	SFT	SINGLE FLOWER TRUSS gene involved in regulation of flowering	Lifschitz et al., 2006
	CLV3	CLAVATA3 key meristematic gene, regulating locule numbers in fruit	Rodríguez-Leal et al., 2017
	PSY1	Phytoene synthase 1 gene involved in the biosynthesis of carotenoid resulting in yellow flesh fruit	Hayut et al., 2017
	ANT1	Anthocyanin mutant 1 gene encodes a Myb TF which involve in increasing anthocyanin content	Čermák et al., 2015
	GAD2, GAD3	Key genes encoding an enzyme glutamate decarboxylase for biosynthesis of γ-aminobutyric acid (GABA) in fruit	Nonaka et al., 2017
	ALMT9	Al-ACTIVATED MALATE TRANSPORTER9 gene involved in decreasing the malate content accumulation in fruit	Ye et al., 2017
	MBP21	MBP21 is a MADS-box protein controlling formation of abscission zone in pedicel	Roldan et al., 2017
	BOP1, BOP2, BOP3	BLADE ON PETIOLE gene reported being associated with early flowering with simplified inflorescences	Xu et al., 2016
	SP5G	SELF-PRUNING 5G gene is a flowering repressor linked involved in the development of day-length-sensitive tomato plant	Soyk et al., 2017
Cucumis sativus	WIP1	WIP1 is a C2H2 zinc finger TF gene involved in development of gynoecious plant	Hu et al., 2017
Actinidia chinensis	CEN	CENTRORADIALIS like gene associated with the development of compact plant with early terminal flowering and fruit development	Varkonyi-Gasic et al., 2019