TABLE 1.

Basal diet for different phases for Experiment 1 and 2.

Ingredient (%)	Starter	Grower	Finisher 1	Finisher 2	Withdrawal
Corn	54.22	57.94	66.52	68.4	68.76
Soybean meal	38.78	34.52	26.06	24.3	24.15
Dicalcium phosphate	1.9	1.72	1.53	1.44	1.38
Limestone	0.95	0.89	0.84	0.82	0.78
Salt	0.31	0.24	0.33	0.24	0.25
Sodium bicarbonate	0.3	0.42	0.29	0.47	0.47
1MHA®84%	0.3	0.26	0.27	0.22	0.18
2Mineral premix	0.2	0.2	0.2	0.2	0.2
L-LYSINE HCL 78%	0.14	0.11	0.21	0.18	0.13
Choline Cl-60%	0.05	0.07	0.09	0.09	0.09
3Coban 90	0.05	0.05	0.05	0.05	0.05
Threonine	0.1	0.05	0.08	0.06	0.03
4Vitamin premix	0.05	0.05	0.05	0.05	0.05
5Sand	0.15	0.15	0.15	0.15	0.15
6Soybean oil	2.5	3.33	3.33	3.33	3.33
Total	100	100	100	100	100
Calculated nutrient content
ME (Kcal/Kg)	3030	3120	3201	3226	3230
CP (%)	23.62	21.83	18.67	17.91	17.78
dLys (%)	1.28	1.15	1.02	0.95	0.91
dMet (%)	0.59	0.54	0.51	0.46	0.43
dSAA (%)	0.95	0.87	0.80	0.74	0.71
dThr (%)	0.87	0.77	0.68	0.64	0.61
dCys (%)	0.36	0.33	0.29	0.28	0.28
dArg (%)	1.44	1.32	1.09	1.04	1.04
Calcium (%)	0.96	0.88	0.79	0.75	0.72
Phosphorus (%)	0.48	0.44	0.39	0.37	0.36

Proximate analysis was done for all the diets and confirmed the nutrient value matched the table values.

¹DL-Methionine Hydroxy Analog Calcium, Novus International, Inc., St Charles, MO, United States. For experiment 2, ∼0.06% was reduced in the basal diet for all phase to account the amount coming from mineral premix.

²Trace mineral premix

• Experiment 1: Added at this rate yields 120 mg manganese (sulfate), 110 mg zinc (sulfate), 35 mg iron (sulfate), 20 mg copper (sulfate), iodate (calcium iodate) 1.25 mg per kg of diet. The premix contained calcium carbonate as carrier and less than 1% mineral oil.

• Experiment 2: For treatments OxF and OxF + ETX, premix added at this rate had 120 mg manganese (sulfate), 110 mg zinc (sulfate), 35 mg iron (sulfate), 20 mg copper (sulfate), iodate (calcium iodate), and 575.4 mg MHA per kg of diet. For treatments OxF + MMHAC and OxF + MMHAC + ETX, premix added at this rate had 40 mg manganese (MINTREX^®), 40 mg zinc (MINTREX^®), 35 mg iron (sulfate), 25 mg copper (MINTREX^®), and iodate (calcium iodate) per kg of diet.

³Active drug ingredient monensin sodium (Elanco Animal Health, Greenfield, IN, United States). For the prevention of coccidiosis caused by Eimeria necatrix, Eimeria tenella, Eimeria acervulina, Eimeria brunetti, Eimeria mivati, and Eimeria maxima.

⁴Vitamin premix: for Experiment 1 and 2, added at this rate yields 11574 IU vitamin A, 4125 ICU vitamin D, 125 IU vitamin E, 3.1 mg vitamin K, 0.019 mg B12, 10.0 mg riboflavin, 68.8 mg niacin, 18.8 mg pantothenic acid, 1.87 mg folic acid, 4.4 mg pyroxidine, 3.13 mg thiamine, 0.188 mg biotin per kg diet. ⁵Sand was used as a filler

• Experiment 1: ETX-Ethoxyquin [SANTOQUIN^® M6 at 188 ppm (Ethoxyquin-125 ppm)]. SANTOQUIN^® M6 has 66.6% ethoxyquin and was added at 0.0188% in the FrF + ETX and OxF + ETX diets replacing the same amount of sand.

• Experiment 2: ETX- Ethoxyquin [SANTOQUIN^® M6 at 188 ppm (Ethoxyquin-125 ppm)]. SANTOQUIN^® M6 has 66.6% ethoxyquin and was added at 0.0188% in the OxF + ETX, OxF + MMHAC + ETX diets replacing the same amount of sand.

⁶Soybean oil was used as source of fat.

• Experiment 1: Oxidized soy oil to contain 225 meq peroxide/kg so that the final starter diet to have ∼5–5.5 mEq/kg diet, and the grower and finisher diets to have ∼7–7.5 meq peroxide/kg for OxF and OxF + ETX, while FrF and FrF + ETX received fresh soybean oil.

• Experiment 2: All diets received oxidized soy oil to contain 225 meq peroxide/kg so that the final starter diet to have ∼5–5.5 mEq/kg diet, and the grower and finisher diets to have ∼7–7.5 meq peroxide/kg.