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. 2021 Apr 6;12:663409. doi: 10.3389/fphys.2021.663409

TABLE 2.

Basal diet for different phases for Experiment 3.

Ingredient (%) Starter Grower Finisher
Corn 55.38 57.70 61.83
Soybean meal 36.32 33.29 28.63
Soybean oil 3.18 4.37 5.31
Monodicalcium phosphate 2.15 1.91 1.70
Limestone 1.02 0.96 0.85
Salt 0.20 0.23 0.24
1Pelex® Dry 0.30 0.30 0.30
2BS Premix 0.20 0.20 0.20
3MHA®84% 0.39 0.33 0.30
L-Lysine HCl 0.30 0.23 0.21
L-Threonine 0.18 0.13 0.10
Sodium bicarbonate 0.25 0.22 0.21
Choline Chloride 60% 0.08 0.07 0.08
4Salinomycin 12% (Sacox) 0.05 0.05 0.05
Total 100.00 100.00 100.00
Calculated nutrient content
ME (Kcal/Kg) 3,000 3,100 3,200
CP (%) 23.0 21.5 19.5
dLys (%) 1.28 1.15 1.02
dMet (%) 0.64 0.57 0.53
dCys (%) 0.31 0.30 0.27
dThr (%) 0.86 0.77 0.68
dArg (%) 1.32 1.23 1.10
Calcium (%) 0.96 0.87 0.78
Total phosphorus (%) 0.84 0.77 0.71
Phosphorus (%) 0.48 0.44 0.39

Proximate analysis was done for all the diets and confirmed the nutrient value matched the table values.

1Pelex® is an advanced-generation, synthetic resin that is effective at low inclusion levels as a livestock feed/pellet binder.

2Trace mineral premix: All treatments received 20 mg iron, and 1.2 mg iodine. In addition to that, CON–ITM received 110 mg zinc sulfate, 6 mg copper sulfate, 120 mg manganese sulfate, and 0.3 mg sodium selenite; MMHAC received 50, 10, and 60 mg of MINTREX® Zn, MINTREX® Cu, and MINTREX® Mn, respectively, and 0.3 mg sodium selenite; MMHAC + Org Se + ETX received 50, 10, and 60 mg of MINTREX® Zn, MINTREX® Cu, and MINTREX® Mn, respectively, 0.3 mg ZORIEN® SeY (A special strain of Saccharomyces cerevisiae enriched with selenium in an organic highly bioavailable form) and 125 mg of ethoxyquin (SANTOQUIN® M6 at 188 ppm) per kg of diet.

3DL-Methionine Hydroxy Analog Calcium, Novus International, Inc., St Charles, MO, United States.

4Salinomycin sodium concentrations ranging from 40 to 60 g/ton (0.0044–0.0066%) for the prevention of coccidiosis in broiler, roaster and replacement chickens caused by Eimeria tenella, Eimeria necatrix, Eimeria acervulina, Eimeria maxima, Eimeria brunetti, and Eimeria mivati.