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. 2021 Apr 6;9:659783. doi: 10.3389/fcell.2021.659783

FIGURE 3.

FIGURE 3

Evaluation of optic nerve regeneration in EXOPACAP38-treated rats after ONC. (A) Experimental timeline. ONC was performed on rats followed by intravitreal injection of EXOs, PACAP38, and EXOPACAP38. After 7 days, the rats were given a second injection. Axons were traced with CTB-conjugated Alexa Fluor 488 at 2 days before animal sacrifice. (B) Laser scanning confocal microscope images showing the regeneration of optic nerve axons at 14 days after injury, divided into the untreated, EXO-treated, PACAP38-treated, and EXOPACAP38-treated groups (scale bar = 100 μm). Optic nerve axons were traced using CTB. Asterisks indicate the ONC site. (C) Quantitative analysis of optic nerve axon number in the range of 100–1,000 μm from the crush site (red asterisks in B) (n = 4; one-way ANOVA on Tukey’s multiple comparisons test,*P < 0.05, **P < 0.01, ***P < 0.001, relative to the untreated group).