Fig. 4. PM attachment to the CW is MYB36-dependent but does not rely on lignin deposition.

a Median and surface view of the endodermal plasma membrane using the marker line pELTP::SYP122-mCitrine before plasmolysis (+H₂O) and after plasmolysis (+Mannitol) at 15 cells after the onset of elongation. WT plants were treated or not from germination with 10 µM piperonylic acid (+PA). White asterisks show the exclusion domain at the CSD. The dashed line represents the contours of the cells before plasmolysis. Arrows show the plasma membrane attachment to the cell wall. Blue asterisks show the plasmolysis generated space where no attachment is observed. Scale bar = 5 µm. “inner” designates the stele-facing endodermal surface, “outer”, the cortex-facing surface. The experiment was repeated three times independently with similar results. b Maximum projection of CASP1-GFP and lignin staining with basic fuchsin in cleared roots from plants grown with or without 10 µM piperonylic acid and subjected to plasmolysis with Mannitol. Scale bar = 10 µm. The experiment was repeated two times independently with similar results.