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Expression of TNF-α, NF-κB and HIF-1α genes in colorectal cancer tissues. mRNA levels of (A) TNF-α, (B) NF-κB, (C) HIF-1α, and (D) TNFR1 in tumor tissues compared with normal tissues. β-actin was used as an internal control. Data (mean ± SE) are representative results derived from a minimum of three independent experiments. *P<0.05 and ***P<0.001. HIF, hypoxia inducible factor.
