Figure 1.

Schematic overview of lentiviral transfer vector plasmid maps

Third-generation self-inactivating (SIN) lentiviral vectors (LVs) were constructed using the CMV promoter driving the expression of the viral transcript (pCCL). The transfer vector contains the packaging signal (ψ), the REV responsive element (RRE), central polypurine tract (cPPT), and a modified Woodchuck post-translational regulatory element (bPRE4). Expression of the reporter gene EGFP was under the control of either the PGK or MND promoter (arrows). The WT1-TCR was under the control of the MND promoter.