Figure 3.

CB and PB CD8⁺ T cells show different immune phenotype and transduction efficiency

(A) Transduction efficiency, as percentage of GFP⁺ cells in CB CD8⁺ T cells (black) compared to PB CD8⁺ T cells (gray) using MND.EGFP. (B) Representative flow cytometry plot of CB and PB CD8⁺ T cells transduced with MND.EGFP. (C) Proportion of naive T (T_n) cells, effector memory T (T_em) cells, and central memory T (T_cm) cells based on the expression of CD45RO and CD62L, in both CB and PB in CD8⁺ fresh, expanded, and transduced T cells. (D) Representative flow cytometry plot of CD45RO-CD62L-expressing cells during the three selected time points (i.e., fresh, expanded, transduced). (E) CD45RA expression in CB (black) and PB (gray) CD8⁺ fresh, expanded, and transduced T cells. (F) Co-inhibitory receptor expression in CB (black) and PB (gray) CD8⁺ fresh, expanded, and transduced T cells. The data correspond to ≥3 independent experiments and are shown as average ± standard deviation; ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗∗∗p ≤ 0.0001.