Figure 7.

miR-320b inhibits tumorigenesis and development of lung cancer in vivo by regulating USP37/CDT1

(A) qRT-PCR evaluating the expression of miR-320b in lung cancer cells. (B) Representative images and results of immunoblotting analysis measuring the protein levels of USP37 and CDT1 in lung cancer cells. (C) Images of xenograft tumors at endpoint (left panel), tumor growth curve (middle panel), and final tumor weight (right panel) reflecting the development of lung tumor in vivo. (D) Representative images of tumor nodules on the lung surface. (E) Immunohistochemistry detecting the expression of Ki67 in tumor tissues from mice in each group (scale bar = 25 μm) (∗p < 0.05, model versus control; #p < 0.05, model + miR-320b agmoir + oe-NC versus model + agmoir NC + oe-USP37; &p < 0.05, model + miR-320b agmoir + oe-USP37 versus model + miR-320b + oe-NC). Data are presented as the mean ± standard deviation. Data between two groups were compared using an unpaired t test. Comparisons among multiple groups were performed using one-way ANOVA with Tukey’s post hoc test. Comparisons over time were performed using repeated-measurements of ANOVA with Bonferroni post hoc test. n = 8.