(A and B) qRT-PCR (A) and western blotting (B) analysis of KANSL2, KANSL3, and KAT8 expression 3 days after transduction of THP-1/cdCas9-KRAB cells with corresponding sgRNAs. In (A), values are normalized to RPLP0 and shown as mean ± SD.
(C) Growth curves of THP-1/cdCas9-KRAB cells transduced with a NegCtrl sgRNA or sgRNAs against KANSL2, KANSL3, or KAT8.
(D and E) Cell cycle (E) and apoptosis (F) analysis 7 days after KANSL2, KANSL3, or KAT8 KD. The values are shown as mean ± SD.
(F and G) Principal-component and non-hierarchical clustering analysis of the RNA-seq (F) and proteomics (G) expression data from NSL complex KD series.
(H) Volcano plots describing expression changes for all genes with a nearby KANSL3 ChIP-seq peak (KANSL3 targets) in NSL complex KD series. Differentially expressed genes are indicated in light blue (q < 0.05).
(I) Venn diagram indicating overlap between downregulated KANSL3 target genes and the list of pan-essential genes from the DepMap project.
(J) The gene set enrichment analysis (GSEA) enrichment plot for GO:0070125 gene set (mitochondrial translation elongation) on the basis of differential protein expression after KANSL2 KD.
(K) CRISPR KO competition assays for the selected NSL complex target genes.
See also Figure S2 and Table S2.