Figure 5.

Spontaneous withdrawal following prenatal morphine (10 and 30 mg/kg) exposure affected wall climbing and face cleaning behavior in spiny mice pups. (a) Male pups on PND-4 from the 10 mg/kg morphine exposed group (N = 12) had a significantly greater number of wall climbs compared to the saline-exposed controls (N = 8) (AUC [#Climbs] – Saline: 57.38 $\pm$ 11.87; 10 mg/kg: 73.00 $\pm$ 8.58; 30 mg/kg: 71.71 $\pm$ 9.35). (b) Similarly, female pups on PND-3, 4, 5 from the 10 mg/kg morphine exposed group (N = 12) and PND-5, 6 pups from the 30 mg/kg morphine exposed group (N = 9) had a significantly greater number of wall climbs compared to the saline-exposed controls (N = 9) (AUC [#Climbs] – Saline: 55.28 $\pm$ 9.69; 10 mg/kg: 80.25 $\pm$ 8.11; 30 mg/kg: 72.20 $\pm$ 7.64). (c) Male pups on PND-1 from the 30 mg/kg morphine exposed group (N = 12) had a significantly greater number of face cleanings compared to the saline-exposed controls (N = 8) and 10 mg/kg morphine exposed group (N = 12) (AUC [#Cleans] – Saline: 4.50 $\pm$ 1.29; 10 mg/kg: 5.04 $\pm$ 1.43; 30 mg/kg: 5.54 $\pm$ 1.82). (d) Female pups on the PND-1 from the 10 mg/kg morphine exposed group (N = 12) had a significantly greater number of face cleanings compared to saline-exposed controls (N = 9) and the 30 mg/kg morphine exposed group (N = 9) (AUC [#Cleans] – Saline: 5.17 $\pm$ 1.43; 10 mg/kg: 9.17 $\pm$ 2.33; 30 mg/kg: 5.60 $\pm$ 1.92). Data are presented as means totals (±SEM) during the PND 0–7 period. An asterisk symbol (∗ = saline vs. 10 mg/kg morphine) or pound symbol (# = saline vs. 30 mg/kg morphine) or nabla symbol (∇ = 10 mg/kg morphine vs. 30 mg/kg morphine) are used to indicate a significant difference between groups, P < 0.05.