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. 2021 Apr 5;2(2):100434. doi: 10.1016/j.xpro.2021.100434
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
Benzonase Nuclease Millipore Sigma E1014-5KU
DBCO-mPEG, 5, 10, or 20 kDa Click Chemistry Tools A118-25, A119-25, A120-25
Dimethyl sulfoxide (DMSO) Sigma-Aldrich D8418-50ML
Ethylenediaminetetraacetic acid (EDTA) disodium salt dihydrate >99.0% Sigma-Aldrich 03677
Gly3-PEG3-Lys(azide)-PEG3-DFO GenScript (custom synthesized) N/A
Glycerol, for molecular biology, ≥99.0% Sigma-Aldrich G5516-100ML
HEPES powder Thermo Fisher, Gibco 11344041
Imidazole, for molecular biology, ≥99% (titration) Sigma-Aldrich I5513-25G
100 mM IPTG solution Sigma-Aldrich 70527-3
Lysozyme Millipore Sigma L6876-1G
MgCl2 Sigma-Aldrich M8266-100G
Na2CO3 powder >99.5% Sigma-Aldrich 223530
Na2HPO4·7H2O Sigma-Aldrich S9390-500G
NaCl, cell culture reagent grade MP Biomedicals, Inc 02194738.5
NaH2PO4·H2O Sigma-Aldrich S3522-500G
Oxalic acid 98% Sigma-Aldrich 194131-250G
Sucrose BioReagent, suitable for cell culture ≥99.5% (GC) Sigma-Aldrich S1888-500G
TraceSELECT HCl Thermo Fisher 60-007-78
Tris base Sigma-Aldrich 10708976001
Triton™ X-100 Sigma-Aldrich T8787-100ML
89Zr oxalate in 1M aqueous oxalic acidSpecific activity approximately 5–10 mCi/μg. 3D-imaging N/A
Yeast extract, for use in microbial growth medium Sigma-Aldrich Y1625-250G
Tryptone, pancreatic digest of casein Sigma-Aldrich T9410-250G
Tissue-Tek OCT compound 4 OZ Andwin Scientific NC9806257
Kanamycin sulfate from Streptomyces kanamyceticus Sigma-Aldrich K1377-1G
ISOVUE®-370 (iopamidol injection 76%) Bracco 0270-1316-95
HisPur™ Ni-NTA Resin Thermo Fisher 88222
Forane (isoflurane, USP) liquid for inhalation Baxter 1001936040
1× Dulbecco’s phosphate-buffered saline (DPBS)w/o CaCl2 and MgCl2 Thermo Fisher, Gibco 14190144
Chelex® 100 sodium form Sigma-Aldrich C7901-100G
Ampicillin Sigma-Aldrich A5354-10ML
Agar, for microbiology Sigma-Aldrich 05040-250G
Bacterial and virus strains
Escherichia coli BL21(DE3) competent cells - Novagen Sigma-Aldrich 69450-3
Escherichia coli (Migula) Castellani and Chalmers (WK6) competent cells ATCC 47078
Experimental models: Organisms/strains
Mouse C57BL/6J, 8 to 12 weeks old Jackson Laboratories 000664
Recombinant DNA
Plasmid pET30b-7M SrtA Addgene 51141
Software and algorithms
OsiriX MD software OsiriX Foundation N/A
ImageJ National Institutes of Health N/A
Mediso Nucline Software Mediso N/A
Other
3M™ Micropore™ surgical paper tape 3M 7000002543
Alcohol prep pads Medique 22133
Autoradiography cassettes Cytiva 45-000-758
BD Lo-DoseTM U-100 insulin syringes 28G 0.5 mL BD 329461
BD Micro-fineTM IV insulin syringe 28G 1 mL BD 329420
BD PrecisionGlide™ single-use needles: regular bevel and regular wall 27G 1¼ in BD 305136
Caution radioactive materials pre-strung tags Research Products International Corp 140050
Clear plastic wrap Fisherbrand 22-305654
Disposable PD-10 desalting column Sigma-Aldrich, GE GE17-0851-01
Disposable base molds Fisherbrand 22-363-554
Intravascular polyurethane tubing 0.005 ID 0.010 OD 25 ft catheter SAI Infusion Technologies PU-025-50
iTLC-SG chromatography paper Agilent SGI0001
Kimberly-Clark Professional™ Kimtech Science™ Kimwipes™ delicate task wipers Kimberly-Clark Professional 34120
pH test strips pH 4.5–10, resolution: 0.5 pH unit Sigma-Aldrich P4536
Phosphor image plate Fujifilm BASMS-2325
Rotating tail injector restrainer Braintree Scientific RTI STD
Shielded syringe carrier, small Biodex 001-182
NanoDrop™ 2000/2000c spectrophotometers∗ Thermo Scientific ND2000CUSCAN
Misionix Sonicator 3000∗ Misonix Inc S-3000
Atomlab™ 500 dose calibrator∗ Biodex 086-330
Auto flow anesthesia system with regulator, induction chamber, beds, breathers, and charcoal filters∗ Euthanex EZAF9000
NanoScan PET/CT∗ Mediso n/a
Chemyx Fusion 100T precision lab infusion syringe pump∗ Thermo Scientific BZA649110
CM3050 S Research Cryostat∗ Leica 14903050S01
1480 Wizard 3-inch gamma counter∗ PerkinElmer 10071799
Storage phosphor screen image eraser∗ GE Healthcare 29187190
Fiber optic illuminator with gooseneck∗ Dolan-Jenner 660000391014
Lighting specialties TEL-AF-3191 infrared heating lamp, 50/60 Hz∗ Cole-Palmer 0305700
Typhoon 7000IP plate reader∗ GE Healthcare 28-9836-18
Radio-TLC scanner∗ LabLogic Dual Scan-Ram