| Reagents | Final concentration | Amount |
|---|---|---|
| Neurobasal (NB) without phenol red | n/a | 485 mL |
| Glutamax I Supplement 100× | 1% | 5 mL |
| B27 | 2% | 10 mL |
| Penicillin/Streptomycin 100× | 1% | 4.5 mL |
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